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RNA-Seq in Mouse Kidney ASDN with MR (NR3C2) Knockout

RNA sequencing (RNA-seq)-based transcriptomic profiling was performed in aldosterone-sensitive distal nephron (ASDN), including connecting tubule and cortical collecting duct tubule, microdissected from kidneys of MR (Nr3c2) knockout (KO) mice.

Nr3c2-flox/Pax8‐rtTA/LC1 mice were used as a doxycycline (DOX)-inducible Nr3c2 gene KO model. After DOX treatment, four groups were prepared to distinguish between K+ diet and MR KO effects: 1) control mice on normal K+ diet (CT-NK) or 2) high K+ diet (CT-HK) and 3) MR KO mice on normal K+ diet (KO-NK) or 4) low K+ diet (KO-LK). RNA-Seq analysis was carried out in the microdissected connecting tubule and cortical collecting duct tubule segments (5-6 mice per group; ~10 fresh ASDN tubules per mouse). [Potassium diet] Normal K, 1% KCl; High K, 2.1% KCl; Low K, <0.003% KCl. [MR/GR binding] Tier1, -1kb stream to +100bp of TSS; Tier2, -3kb stream to +1kb of TSS; Tier3, -5kb upstream of TSS and gene body. FDR(False discovery rate, BH-corrected) < 0.05 is considered as significantly changed genes.

Raw sequence data available at NCBI GEO GSE269263. Please contact to Hyun Jun Jung (hynjn.jung@gmail.com) or Paul A. Welling (pwelling1@jhmi.edu, @Welling Lab).