Comprehensive Transcriptome Database of Mouse Kidney DCT

This is a comprehensive transcriptome database of Distal Convoluted Tubule (DCT), generated from freshly isolated early DCTs (DCT1). This database provides an interactive platform to explore high-resolution transcriptome profile of the early DCTs (Pvalb-positive) of the mouse kidney.

This database provides sex differences in transcriptome profiles of DCTs isolated from female and male kidneys and time-dependent transcriptional changes in the DCT after furosemide treatment.

Available Datasets

Explore Interactive Database (Shinyapps)

Kidney DCT Gene Atlas

Transcriptomic difference of the DCT in Female vs Male

Mice with Pvalb-Cre-dependent expression of the fluorescent protein were developed and fluorescence-positive DCT (early DCT; DCT1) segments were dissected from the kidneys.
Microdissected DCTs from female and male kidneys were analyzed by RNA-Seq (~5 tubules per sample; 6-7 mice per sex).
Differentially expressed genes between female and male (FDR < 0.05) were analyzed.

Download processed data: Download excel

Access to raw data: NCBI GEO (GSE269410)

Time-course transcriptomic changes in the DCT after furosemide treatment

Mice with Pvalb-Cre-dependent expression of the fluorescent protein were fed with furosemide-containing diet for overnight (12h), 24h, 48h, and 96h (4 females and 4 males per time point).
Fluorescence-positive early DCT (early DCT; DCT1) segments were dissected from the kidneys and were analyzed by RNA-Seq (~5 tubules per sample).
Differentially expressed genes over time after furosemide treatment (FDR < 0.05) were analyzed.

Download processed data: Download excel

Access to raw data: NCBI GEO (GSE269409)

Citation

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